技術概述
Applied Biosystems (ABI) 3730xl DNA Analyzer
- 96-capillary array provides high-throughput sample processing
- Higher optical sensitivity and advanced polymers can provide higher-quality sequencing data.
- Optimized polymers also increase the productivity without compromising the results.
- High accuracy and analysis of DNA sequence up to 1,000 bp.
- A wide variety of sequencing and fragment analysis applications, such as DNA sequencing, microsatellite analysis, and DNA methylation detection, etc.
Dye Set Selection
Currently CPOS utilizes filter set G5 and the ABI PRISM Linkage Mapping Set DS-33 with the following fluorescent dyes:
| Dye | Colour |
| 6-FAM | Blue |
| VIC | Green |
| NED | Yellow |
| PET | Red |
| LIZ (the size standard) | Orange |
尺寸範圍
CPOS routinely provides GeneScan™ 500 LIZ® as size standard.
It is suitable for sizing DNA fragments in the 35-500 nucleotides range.
工作流程
樣品製備和提交
Successful Analysis Requires High Quality DNA
樣品製備
Purify the samples from excessive salts, organic solvents and residual ethanol.
(Commercial DNA extraction/PCR products purification kits are commonly used for this purpose.)
Submit at least 2µl of DNA samples.
Seal the plate with adhesive film and then enclose them in a plastic bag.
Protect GeneScan product from light.
樣品提交
請參考 “How a user makes a service request (Sanger Sequencing)” section for further information.
- 透過以下方式提交服務請求 iLab.
- 填寫 GeneScan Sample Submission Form.
- Use one form per plate
- Fill in ALL information clearly or job will be delayed
- Expected read length for each sample is required for data QC
- Submit GeneScan Sample Submission Form as attachment through iLab.
- Submit samples to the CPOS reception counter (6-02) with hard copy of GeneScan Sample Submission Form.
*Staple the form outside the plastic bag.
Please refer to Sanger page for instructions on submission via drop-off boxes 和 courier delivery.
Sample Collection Time
Monday to Friday (except public and university holidays)
9:30am to 12:30pm
2:00pm to 5:30pm
Sample ID Submission
Fill in the electronic GeneScan Sample Submission Form for sample ID submission.
Give each sample a unique name.
Submit GeneScan Sample Submission Form as attachment through iLab.
Name the file using the format “date(yyyymmdd)_lastname_firstname”, e.g. 20080616_chan_peter
數據收集
GeneScan results are sent to clients as zipped file through 線上資料傳遞系統 (ODDS), notification will be sent to the email provided on the service request form upon data delivery.
Our current turn-around time is 1-2 working days after sample submission. However, the turn-around time could be longer at times due to unforeseeable circumstances and busy sequencing schedules.
We recommend using the software GeneMapper v3.7 from ABI for microsatellite analysis.
This software is available on one of our user data analysis workstations (User PC#2) in Room 6-01 at CPOS.
Clients may make advanced bookings for the workstation via our online resource booking system (ORBS) or at our reception counter (Room 6-02).
服務條款
Re-run Policy
Failed GeneScan runs will be subjected to in-house QC auditing.
If the failure is determined to be instrument-associated, e.g. gel anomalies, samples will be automatically re-run at no extra charge to the client.
If the failure is determined to be sample-associated, no re-run will be performed. Instead, CPOS can provide consultations including a suitable protocol that may improve the GeneScan results.
Our goal is to give you the highest quality of microsatellite analysis data possible.
聯絡我們
陳女士，喬伊斯
謝女士，克里斯汀
